The optimal TTC dose and its chemical reduction level during soil dehydrogenase activity assay
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| Authors: | Agnieszka Wolińska1, Magdalena Zapasek1, Zofia Stępniewska1 1Department of Biochemistry and Environmental Chemistry, The John Paul II Catholic University of Lublin Al. Kraśnicka 102, 20-718 Lublin, Poland |
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| Abstract : | 2,3,5-triphenyltetrazolium chloride (TTC) is a dye largely used for determination of microbiological activity in soil samples by dehydrogenase assay. TTC is colourless in the oxidised form and red when reduced, due to formation of the product 1,3,5-triphenyl formazan (TPF). In this study, different doses of TTC ranging from 0.2 to 3.0% were added to six different soil types with the aim to verify the effect of chemical TTC reduction and to optimise the substrate dose in order to prevent a toxic effect on microorganisms. Regarding the technical simplicity and cost-effectiveness, the TTC assay is commonly recommended as an efficient method for determination of soil biological activity, provided that an appropriate concentration is applied, depending on the soil type. The lowest TTC levels of 0.2 and 0.5% are recommended for Albic Luvisol and Eutric Fluvisol, respectively, and the highest (2%) for Brunic Arenosol and Haplic Luvisol soils. It has also been demonstrated that DHA has biological origin in more than 90%, whilst chemical TTC reduction accounts for less than 10%, and therefore does not interfere with the correctness of the results obtained. |
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| Keywords : | dehydrogenase activity, soil, TTC dose, chemical TTC reduction | ||||||||
| Language : | english | ||||||||