Water-soluble polysaccharides from Opuntia stricta Haw. fruit peels: recovery, identification and evaluation of their antioxidant activities
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| Authors: | M. Koubaa1, A. Ktata2, F. Barba3, N. Grimi1, H. Mhemdi1, F. Bouaziz2, D. Driss2, S. Chaabouni2,4 1Sorbonne Universités, Université de Technologie de Compiègne, Laboratoire Transformations Intégrées de la Matière Renouvelable (UTC/ESCOM, EA 4297 TIMR), Centre de Recherche de Royallieu, B.P. 20529, 60205 Compiègne Cedex, France 2Enzyme Bioconversion Unit (UR13ES74), National School of Engineering, P.O. Box 1173-3038, Sfax University, Tunisia 3Faculty of Pharmacy, Nutrition and Food Science Area, Universitat de València, Avda. Vicent Andrés Estellés, s/n. 46100 Burjassot, Spain 4Common Service Unit of Bioreactor coupled with an ultrafilter, National School of Engineering, P.O. Box 1173-3038, Sfax University, Tunisia |
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| Abstract : | A b s t r a c t. Opuntia stricta Haw. is considered as one of the most common cactus plant growing in Tunisia. Extracting valuable compounds from its fruit peel, considered as by-product, is drawing more and more attention, making it on the verge of commercialization. Water-soluble polysaccharides were extracted from Opuntia stricta Haw. peels, and their chemical composition assessed using thin layer chromatography. The antioxidant activities of the extracted polysaccharides were assessed using 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity, total antioxidant activity and reducing power capacity. The extraction yield of water-soluble polysaccharides was 7.53±0.86%. The chemical composition revealed the presence of rhamnose, arabinose, glucose, mannose, galactose and galacturonic acid. The infrared spectroscopic analysis showed a similar structure to that of Opuntia ficus-indica polysaccharide peels. Additionally, the extracted polysaccharides exhibited high antioxidant activities. In fact, the free radical scavenging activity (half inhibition concentration = 6.5 mg ml-1 with 94.9% inhibition at 50 mg ml-1), the total antioxidant activity (100 μg ascorbic acid equivalent at 50 mg polysaccharides) and the reducing power capacity (absorbance 700 nm = 0.7 at 50 mg ml-1), appeared to be interesting compared to natural and synthetic antioxidants. Therefore, water-soluble polysaccharides from Opuntia stricta Haw. fruit peels could be a natural |
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| Keywords : | Opuntia stricta Haw. peels, polysaccharide extraction, water-soluble polysaccharides, antioxidant activity | ||||||||||
| Language : | English | ||||||||||