Laboratory of Microscopy
Equipment
- CLSM allows for micro-structure observation of samples surfaces and 3D imaging. Images are taken only from thin confocal layer, thus images are usually very contrastic.
- Main features: Two lasers: Ar 488 nm, HeNe 543 nm, 3 channels (two confocal, one optical), high resolution; XY, XYZ, XYZT scanning modes.
- AFM allows for nano- and micro-structure observation with analysis of mechanical properties at these scales in air or liquid of biological samples.
- Main features: Contact, tapping, force volume modes; Harmonix mode for mechanical properties imaging; system for perfusion; temperature control, integration of optical and AFM view .
- Structural identification and analysis of chemical compounds, mapping of chemical compound distribution in samples.
- Analysis of multi-molecular compounds; analysis of cellulose crystallinity.
- Distribution of active substances in pharmaceutical tablets; chemometric polymer analysis.
- Computer analysis of 2D and 3D images.
- 2D and 3D image transformation, and analyses, including colour, size and orientation of represented objects.
- Macroscope is a microscope using the dark field view. It is designed for observation large sample areas. Sample must be transparent.
- Main feature: field of view up to~8x6mm. Useful for evaluation of samples heterogeneity.
Confocal Laser Scanning Microscope (CLSM) - FluoView300
Atomic Force Microscope (AFM) - BIOSCOPE CATALYST II
Fourier-transform infra-red spectroscopy (FTIR)/Raman spectroscopy system
The system includes Raman disperse spectrometer with 532 nm laser, Fourier-transform infra-red Raman spectrometer
with 1064 nm laser, IR spectrometer and UV-VIS spectrometer.
Examples of use:
Aphelion Dev. 4.0 image analysis software
Microtome - LEICA RM2155
Microtome is a tool for slicing fixed samples with high precision (< 1 micrometer).
Main features: Cutting accuracy <1 micron, fully programmable; possibility to use different blades: glass, stainless steel, sapphire.
Vibratome - LEICA VT 1000S
Vibratome is useful for slicing soft materials like plant and animal tissues. Surface has decent quality for CLSM or macroscope.
Main features: Adjustable thickness (with 1 micron step), cutting speed and oscillations; batch for sample allowing cutting in buffers; fully programmable; possibility of use simple razors blades.
Macroscope
