Laboratory of Biochemistry
Expertise
Sample preparation
- Isolation of cell walls from different plants
- Sequential extraction of polysaccharides from cell walls:
- Pectin fractions: WSP, CSP,ASP
- Hemicelluloses fractions
- Cellulose
- Extraction of natural compounds with ASE 150 with solvents: ethyl alcohol, acetone, water
- Sample lyophilisation
- Dry sample grinding to the particle size of c.a. 6µm.
- Microbiological medium and small laboratory equipment sterilization
Traditional chemical analyses
- Dry mass marking
- Acidity
- pH
- Extract contents
- Starch contents, iodine test, enzyme method, polymetric method
- Marking the level of pectin estrification with a spectrophotometric method
- Enzyme activity - a spectrophotometric method
- Polygalacturonase PG
- Alfa – L – arabinofuranosidase (Alfa – L –Af)
- Beta – galactosidase (Beta – Gal)
- Pectin methylesterase PME
Flow analysis
- Marking the contents of galacturonic acid in water solutions (1– 100 ppm C6H12O6)
- Marking the contents of calcium in water solutions (1-100 ppm Ca)
- Marking total nitrogen in samples after mineralization (0.1 – 10 ppm N)
FT Raman and FT IR
- Identification of organic and inorganic compounds
- Defining crystallization level of cellulose in cell walls
Granulometric analysis of powders
- 50µm, 100µm, 500µm, 1 mm, 2mm, 4mm.
Analytical services - microbiology
- Qualitative and quantitative marking of fruit and vegetable microflora,
- Gluconacetobacter xylinus bacteria breeding.
contact: Justyna Cybulska
