Laboratory of Molecular and Environmental Microbiology
Equipment
- 4-capillary analyzer for electrophoresis separation of DNA fragments using capillary metod for sequencing and fragment analysis samples
- Basecalling accuracy for sequencing – 98,5%
- Basecalling accuracy for fragment analysis – 99,99%
- Software for:
- data collection and reporting,
- microbial identification of bacteria and fungi based on sequence of rDNA,
- Validated libraries of sequences of known microorganisms for identification of unknown microorganisms
- Thermal cycling system based on Peltire system for 96-well plates or strip-tube – volume 0,1 ml
- System with camera for fluorescence image registration
- Detection in 5 chanels: -FAM/SYBR Green I, -VIC/JOE, - NED/TAMRA/Cy3, - ROX/Texas Red, - Cy5
- Passive reference dyes: ROX Or Any calibrated dye
- Thermal cycler with 96-well block (format 96 x 0,1 ml) made with 6 different areas controlled Rusing Peltier effect
- Homogeneity temperature in the block +/-0,5°C
- Posibility of regulation temperature between areas, as 4°C, and posibilty of setting the same temperature in all areas of the block
- Temperature range from 4,0°C to 99,9°C with posibility setting accuracy for 0,1°C
- System for DNA mutation analysis using DGGE technique (denaturing gradient gel electrophoresis)
- System consists of temperature control module, two electrophoresis tank, one with ceramic cooling fingers and core
- The system allows of two gels electrophoresis
- The control module contains the stirrer for buffer mixing ans heater for temperature control 45-70°C
- Power with voltage
- Aparatus for nucleic acids electrophoresis in agarose gel
- Systems for separation of DNA restriction digestiong and polymerase chain re action-amplified fragments
- Systems with UV-transparent plastic trays
- Power with voltage power supply
- Work time from 1 to 60 second with increase as 1 s
- Speed 4.0–6.5 m/s, with increase as 0.5 m/s
- Adapter with 24 positions for 2 ml tubes
- Cryogenic adapter holds 24 2-ml tubes
- Spectrophotometer UV/VIS for measurement samples in micro-volume
- Sample volume – 0,5 μl
- Range of wavelength – 190-840 nm
- Measurement without cuvette, micro-cuvette and tips
- Range of DNA concentration measurement: 2-15 000 ng/μl
- Time of measurement cycle ≤ 5 sek
- System for vizualization, saling and analysis of fluorescent and colorimetric labeled samples
- System with mini-darkroom with UV transilluminator and white light epi-illumination
- Transilluminator equipped with UV lamps (302 nm UV-B)
- System with filters for using ethidium bromie and Sybr-Green
- Software can be used to image analysis, automatic identification of bands and dendrograms preparation
- System for automatic identification of bacteria: gram (-), gram (+), Bacillus, anaerobic bacteria, yeast and fungi
- System for microbial identification on 96-well microplates with database containing 2000 of bacteria, yeast and fungi species
- System with microstation reader for 96-well plates for identification and characterisation of microorganisms (anaerobic bacteria, yeast, fungi)
- System with automatic station of bacteria identification (gram (+), gram (-) ), for incubation, reading and results analysis for 50 microplates 96-well (temperature incubation range from 22 to 45⁰C)
- System equipped with turbidimeter, aparatus for Gram method staining, apparatus for microaerofiles and anaerobes incubation, optical microscope
- Aparatus with function of fluorimeter, photometric system and
- Detection methods: absorbance with light source – UV xenon flashlamp, fluorescencje
- Absorbance from 230 nm to 1000 nm
- Reader for plates from 6 to 384-well plates
- Read time of 96 –well plate – 20 sec
- Reader with quarto plate for 16 measurement of DNA, RNA and protein concentration in 2 ml
- Aparatus with shaker
- The machine for media and diluents preparation in the range from 1 to 9 l
- The machine equipped with magnetic stirrer – continuous work 40 rpm
- Sterilisation temperaturę in the range 95-125⁰C
- Sterilisation time in the range 1-180 min.
- Dispensing temperature 25-80⁰C
- Constant temperaturę during suplement addition
- Dispensing speed – to 200 ml/min
- Automatic distribution nozzle
- Range of dilution: from 1:2 to 1:99
- Weight of the sample 0 – 2500 g
- Flow rate: to 650 dishes during 1 hour
- Dispensing volume in the range from 1 to 30 ml
- Manual dispensing from 1 to 1000 ml
- Equipped with cooling system
- Dispensing in sterile UV-chamber
- Opening time of the plate < 3 sec.
- Medium mixing during dispensing
- Automatic counting of colony from Petridish (90mm) from pour, deep and spiral plate colony
- Posibility of colony counting from different type of agar
- Possibility of areas create from which colony will be counted
- Automatic colony classification depending on the color
- Automatic separation of overlapping colony
- Possibility of results archiev
- Automated pipetting system to dispense quantities of liquid in the volume 1 µl to 1000 µl
- System for 24, 48, 96 i 384-well plates and 0,2; 0,5; 1,5 oraz 2,0 ml tubes
- Capacity 75 l
- Range of temperature: 105-138oC
- Autoclave chamber made of stainless steel
- Maximum capacity - 3 kg
- Amplitude range from 0,2 to 3,0 mm
- Dry and wet sieving
- Capacity 100 l for minute
- For standard Petridish 90 mm
- Possibility of delayed working: 00:00 do 00:59
Genetic Analyzer 3130
Specifications:
Thermal cycler for Real-Time PCR 7500 Fast
Specifications:
Gradient thermal cycler 9901 Veriti 96 well Fast Thermal Cycler
Specifications:
The DCode Universal Mutation Detection System for DGGE analysis
Specifications:
Sub-Cell GT Agarose Gel Electrophoresis System
Specifications:
Aparatus for DNA extraction
Specifications:
Spectrophotometer for DNA concentration analysis NanoDrop2000
Specifications:
Gel documentation system
Specifications
Automatic system for Fast microorganisms identification : Turbidimeter, Microstation, OmniLog, Microscope, Anoxomat, AeroSpray
Specifications:
Microplates counter INFINITE M200PRO
Specifications:
The autopreparator and sterylizator of microbiological media Masterclave 09
Specifications:
Automatic diluter Dilumat S
Specifications:
Automatic Petri dish filling system APS One
Specifications:
Colony counter Easy Count2 7510/AES
Specifications:
Pipetting stadion for PCR
Specifications:
Autoclave FVG2
Specifications:
Automated screening AS200 Control
Specifications:
Sammler for microbiologiacl analysis of the air Sampl’air
Specifications:
