Laboratory of Molecular and Environmental Microbiology
Expertise
Preparation and storage of samples
- Preparation and dispensing of microbiological culture media
- Sterilization of microbiological media, small glass and laboratory equipment
- Environmental samples homogenization
- Preparation of inoculating fluids and diluter used in microbiological analyses
- Preparation of microscope slides and automatically stain using Gram method
- Preparation and culturing of fungi in microcultures
- Storage of bacterial and fungal strains in microbanks in low temperature freezer (to -85 ° C)
Analyses based on molecular biology techniques
- Selection and optimization of the DNA isolation methods from environmental samples, mycelium and bacteria and yeast cells
- Identification of bacteria based on 16S rRNA sequencing analysis
- Identification of fungi based on D2 fragment sequencing, located within the gene encoding the large subunit of the ribosome (LSU)
- Analysis of genetic profile of microorganisms by amplified fragment length polymorphism (AFLP)
- Phylogenetic analysis of bacterial and fungal strains isolated from environmental samples
- Genetic diversity of bacterial and fungal communities using polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) and terminal restriction fragment length polymorphism (t-RFLP)
- Detection of microorganisms, especially heat resistant fungi by the moloecular biology methods (PCR, qPCR, LAMP)
- Detection and expression analysis of genes encoding cellulose degradation enzymes (PCR, qPCR, qRT-PCR)
Analyses based on BIOLOG System
- Identification and characterization of metabolic profile of fungi (FF plates)
- Identification and characterization of metabolic profile of bacteria(GEN III plates)
- Identification and characterization of metabolic profile of yeast (YT plates)
- Identification and characterization of metabolic profile of anaerobic microorganisms(AN plates)
- Functional microbial diversity evaluation based on characterization of soil metabolic profile (ECO plates)
- Microorganisms analysis by phenotype microarrays method (PM plates)
- Evaluation of microorganisms sensitivity to chemical compounds, plant extracts and fungicides (MT2 plates)
- Assessment of various compounds, waste and products degradation by microorganisms (MT2 plates)
Classical microbiological methods
- Total number of bacteria and fungi evaluation in environmental samples
- Evaluation number of heat resistant fungi and their isolation from environmental samples
- Qualitative and quantitative evaluation of proteolytic, pectinolytic, cellulolytic and amylolytic, microorganisms, ammonifying and nitrifying bacteria in environmental samples
- Identification of fungi based on macro- and microscopic observations
- Soil respiratory activity evaluation
- Assess the sensitivity of fungi to fungicides
- Evaluation of the microbiological air contamination using air sampler
- Evaluation of water microbial quality using membrane filter method
Classical biochemical analyses
- Enzymatic activity evaluation of bacterial and fungal strains (cellulase, pectinesterase, polygalacturonase, protease, lipase)
- Soil enzymatic evaluation using spectrophotometric methods (dehydrogenases, protease, urease, acid and alkaline phosphatase, β-glucosidase)
- Evaluation of ammonification and nitrification rate in soil environment
- Evaluation of soil enzymatic activity using fluorimetric methods (leucine aminopeptidase, esterase, acid and alkaline phosphatase, arylosulphatase, β-glucosidase)
